Differential pattern of Cu/Zn superoxide dismutase isoforms in relation to tidal spatio-temporal changes in the blue mussel Mytilus edulis.
Identifieur interne : 000A29 ( France/Analysis ); précédent : 000A28; suivant : 000A30Differential pattern of Cu/Zn superoxide dismutase isoforms in relation to tidal spatio-temporal changes in the blue mussel Mytilus edulis.
Auteurs : Julie Letendre [France] ; Bastien Chouquet ; Béatrice Rocher ; Hélène Manduzio ; François Leboulenger ; Fabrice DurandSource :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [ 1532-0456 ] ; 2008.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Isoenzymes, Superoxide Dismutase.
- enzymology : Digestive System, Gills, Mytilus edulis.
- Animals, Ecosystem, Periodicity, Seawater.
Abstract
Inducible antioxidant defences in marine organisms such as mussel bivalves are commonly used as biomarkers of pollutant-induced oxidative stress and their variations proposed as one of the biological effect measurements for assessment of contamination impact in aquatic environments. Among them, the copper/zinc superoxide dismutases (Cu/Zn-SODs) are metalloenzymes which play a key role in the protection of cells in case of oxidative stress. In order to observe possible variations of an antioxidant response in relation to tidal oscillations, the copper/zinc superoxide dismutase activity (Cu/Zn-SOD) was characterized in the digestive gland and gills of blue mussels sampled at high and low shore throughout the tidal cycle. Determination of SOD activity was performed on gels after isoelectro-focusing, allowing the revelation of three isoforms. In both tissues, high-shore mussels exhibited a higher level of total SOD activity than low-shore mussels. During emersion, a decrease of total SOD activity appeared in digestive gland for both groups. In high-shore mussels, the less acidic form contributed to 75% of the total activity, the second one to 20% and the more acidic one to 5% in both tissues before air exposure. During emersion, the relative contribution of the three isoforms to the total activity was markedly changed with a significant decrease in intensity of the first isoform and parallel increases in the two other ones. After re-immersion a progressive recovery of proportions of SOD isoforms was observed. In low-shore mussels, the relative contribution of the three isoforms to the total SOD activity showed similar changes. The observed variations could correspond to changes in the redox status of the mussels during tidal oscillations.
DOI: 10.1016/j.cbpc.2008.05.012
PubMed: 18619914
Affiliations:
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pubmed:18619914Le document en format XML
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Julie.Letendre@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Ecotoxicologie, Milieux Aquatiques (LEMA), UPRES-EA 3222, IFRMP 23, Université du Havre, 25 rue Philippe Lebon, BP 540, 76058 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName><orgName type="university">Université du Havre</orgName></affiliation></author><author><name sortKey="Chouquet, Bastien" sort="Chouquet, Bastien" uniqKey="Chouquet B" first="Bastien" last="Chouquet">Bastien Chouquet</name></author><author><name sortKey="Rocher, Beatrice" sort="Rocher, Beatrice" uniqKey="Rocher B" first="Béatrice" last="Rocher">Béatrice Rocher</name></author><author><name sortKey="Manduzio, Helene" sort="Manduzio, Helene" uniqKey="Manduzio H" first="Hélène" last="Manduzio">Hélène Manduzio</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Durand, Fabrice" sort="Durand, Fabrice" uniqKey="Durand F" first="Fabrice" last="Durand">Fabrice Durand</name></author></analytic><series><title level="j">Comparative biochemistry and physiology. 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Among them, the copper/zinc superoxide dismutases (Cu/Zn-SODs) are metalloenzymes which play a key role in the protection of cells in case of oxidative stress. In order to observe possible variations of an antioxidant response in relation to tidal oscillations, the copper/zinc superoxide dismutase activity (Cu/Zn-SOD) was characterized in the digestive gland and gills of blue mussels sampled at high and low shore throughout the tidal cycle. Determination of SOD activity was performed on gels after isoelectro-focusing, allowing the revelation of three isoforms. In both tissues, high-shore mussels exhibited a higher level of total SOD activity than low-shore mussels. During emersion, a decrease of total SOD activity appeared in digestive gland for both groups. In high-shore mussels, the less acidic form contributed to 75% of the total activity, the second one to 20% and the more acidic one to 5% in both tissues before air exposure. During emersion, the relative contribution of the three isoforms to the total activity was markedly changed with a significant decrease in intensity of the first isoform and parallel increases in the two other ones. After re-immersion a progressive recovery of proportions of SOD isoforms was observed. In low-shore mussels, the relative contribution of the three isoforms to the total SOD activity showed similar changes. The observed variations could correspond to changes in the redox status of the mussels during tidal oscillations.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement><orgName><li>Université du Havre</li></orgName></list><tree><noCountry><name sortKey="Chouquet, Bastien" sort="Chouquet, Bastien" uniqKey="Chouquet B" first="Bastien" last="Chouquet">Bastien Chouquet</name><name sortKey="Durand, Fabrice" sort="Durand, Fabrice" uniqKey="Durand F" first="Fabrice" last="Durand">Fabrice Durand</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name><name sortKey="Manduzio, Helene" sort="Manduzio, Helene" uniqKey="Manduzio H" first="Hélène" last="Manduzio">Hélène Manduzio</name><name sortKey="Rocher, Beatrice" sort="Rocher, Beatrice" uniqKey="Rocher B" first="Béatrice" last="Rocher">Béatrice Rocher</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Letendre, Julie" sort="Letendre, Julie" uniqKey="Letendre J" first="Julie" last="Letendre">Julie Letendre</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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